Nutrition Macronutrients

Sustamine: a new supplement for cellular hydration

Sustamine: a new supplement for cellular hydration

by in Nutrition - Macronutrients

last updated: September 04, 2015

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Alanyl-glutamine, better known as sustamine, is a dipeptide which binds glutamine and alanine, resulting in a very stable form with a high level of solubility: let's learn about its benefits

After many years in the pharmaceutical and nutraceutical world, which includes supplements, there are fewer and fewer elements or discoveries that really get me excited or are of particular interest unrelated to my professional activities.

One day the president of Yamamoto Nutrition Italia sent me some supplements and raw materials to evaluate for their launch on the Italian market.

Some of these aroused my interest and approval; I had already seen one of these mentioned in scientific literature, and with positive results.

I'm talking about "alanyl-glutamine", better known commercially as "Sustamine".

Anyone who is involved in the fitness and sports world and uses supplements knows about its "cousin" glutamine, but this relative, at least in Italy, is still very little known.

It is a dipeptide that binds glutamine to alanine, resulting in a form that is very resistant to heat and acids, and presents a high level of solubility.

What is Alanyl-Glutamine?

Starting from its base, or a quick biochemical "review", alanine is a nonessential amino acid, while glutamine is considered "partially" essential; both are involved in the exchange of nitrogen between tissues and in the production and storage of glucose.

Chemical formulas of the amino acids L-Alanine and L-Glutamine

Chemical formulas of the amino acids L-Alanine and L-Glutamine

What is fundamental is the role they play in intracellular hydration; they are of particular interest for their use in intense workouts and other forms of physical stress.

Having said this, it seems that they act in a very similar manner. But more than anything else, I'd define their action as being rather synergistic, especially if we consider that alanine is the most important amino acid involved in the transportation of nitrogen from muscles to the liver.

Once they reach the liver, the carbon fraction of the amino acid skeleton is converted into glucose (a process called the glucose-alanine cycle).

And what has glutamine got to do with it?

Glutamine is the first "carrier" in cells of the nitrogen derived from the catabolism of proteins and it stimulates GLP-1 (glucose-like peptide-1), thus it helps maintain circulating glucose levels as well as promotes the synthesis of the same.

But the main factor that arouses my (and not only) interest is how it improves cellular hydration. Let's see how:

Alanine seems to influence hydration and cell volume by raising the concentration of intracellular potassium, dragging water inside the cell. Glutamine helps keep the equilibrium of the acid-base balance (a particularly popular subject in recent years).

But what has glutamine got to do with it?

Well, muscles produce lactic acid in training, which passes into the bloodstream and acidifies it, giving us that potentially pleasant feeling related to pump, which can then become annoying or a hindrance in certain sports performances.

The increase of lactic acid induces a decrease of pH, and renal glutamine consumption can be significant, which then indirectly raises the percentage of bicarbonate.

After the newly synthesised bicarbonate has reached the blood, it serves as a buffer by binding to the blood's acidic portion in order to make the pH neutral and restore the acid-base balance.

Why use it?

During particularly intense workouts, typical of bodybuilders and very serious physically stressful situations, the muscles release both amino acids in substantial quantities. If these concentrations are not restored, not only will the muscles themselves suffer, also performance, recovery, the immune system and other biological functions will suffer.

Sustamine and weightlifting

That's why, after having clarified where and how they synergistically carry out their functions, this dipeptide "combination" finds its best application with bodybuilders and athletes who perform intense and also prolonged activities that require a marked anti-catabolic action to protect muscles, thereby favouring protein and glucose synthesis as well as giving cells the chance to remain hydrated, and therefore in an anabolic state.

The benefits of Alanyl-Glutamine

The benefits of taking a dipeptide instead of a greater polypeptide chain relate to the aforementioned resistance to heat and acids, and the vastly improved solubility compared to glutamine thanks to the improved efficiency in its use.

Chemical formula of sustamine

Chemical formula of sustamine

Glutamine is rapidly degraded into ammonium when placed in solution and is barely soluble, while alanyl-glutamine is instead incredibly soluble.

This is all because where free form amino acids compete for their uptake with highly selective amino acid transporters, dipeptides are much more easily absorbed through much less selective carriers (PEPT1).


In animal models, alanyl-glutamine has led to an increase in weight and nitrogen retention, while decreasing muscle catabolism and leucine oxidation, and improving the intestinal flora environment.

And here I must note and add to the list of glutamine's important qualities its role as intestinal fuel and its support to the defensive role of the mucosa. But the beneficial role that reigns supreme is the cellular hydration provided by its extreme solubility and stability, and as I mentioned this has already been clearly documented.
Doctors Roger Harris and Jay Hoffman ascertained that the concentrations of glutamine in blood were almost 60% higher in subjects who took alanyl-glutamine than in those who took the free form of glutamine, which then became a total level of plasma glutamine of 126%.
Another interesting finding of the studies is that the regular consumption of alanyl-glutamine appears to increase the levels of muscle glutamine much more than the regular consumption of free form glutamine.

What are its applications?

The main areas of application for alanyl-glutamine are the same as with glutamine (please see my article on its functions):

  • anti-catabolic/cellular hydration actions
  • favours protein and glycogen synthesis
  • supports the intestinal mucosa, and thus the immune system

Sustamine and cycling

Adding alanyl-glutamine to an oral rehydration solution or beverage seems to significantly increase the absorption of water and electrolytes at a cellular level, compared to the addition of L-glutamine or glucose.


This factor is already interesting. In a study by Hoffman and associates, the researchers concluded that the increase in the performance of university-level cyclists that emerged was due to the increase of water and electrolyte absorption, which was much more effective after having taken a Sustamine-based solution.

Those who took Sustamine were able to delay the onset of fatigue and generate only a modest level of dehydration. In this study, Hoffman's team also found that 1g of alanyl-glutamine in 500ml of water can maintain the quality of athletic movements and visual reaction time in a better way in athletes.

When the dose of the dipeptide was doubled in 500ml of water, fatigue decreased but there was no improvement in performance and reaction time. There is a good deal of literary material, which is quite interesting and promotes its use with high marks, (for example the study in the field of clinical nutrition) but must we consider the need for further investigations?

For example, there are still no studies on humans relating to the use of oral alanyl-glutamine to increase an athlete's muscle hypertrophy or strength; it would also be very interesting to study its effects during phases of high workload levels, low carb diets, low hydration levels and/or a combination of all these.

When should you use sustamine?

We all know the different roles that glutamine plays in various fields of application, and sustamine does not substantially deviate from these, it essentially adds a huge "plus" with its stability, and thus its ability to reach "where we want" in higher concentrations in order to best carry out its functions.

Sustamine and swimming

Its consumption on training days before and during exercise should be suggested for helping cellular hydration, electrolyte transport and the metabolism of glucose and branched amino acids.

After exercise it can be useful for supporting recovery, especially in phases of caloric and/or carbohydrate reduction.

On rest days it is instead recommended to fraction intake throughout the day, preferably along with a minimum intake of carbohydrates to optimise glycogen concentrations.
Alanyl-glutamine does not need to be cycled.

There is also evidence that chronic intake, compared to a single high dose, results in an increase in the concentration of glutamine in the muscles compared to those who take it in free form.

Having said that, I invite you to read the various scientific reviews. Most likely with this form of glutamine, we will guarantee the highest expression of the activities of dipeptides, in particular the benefits it offers for absorption, all obviously favouring improved efficacy.

Interesting new supplement on the horizon? I would say so.



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